
As an example, a styled plot can be retrieved by clicking this link, which represents this hyperlink: The URL stores all the user-defined settings and can be used to retrieve the stored layout. This will provide a URL that can be stored or bookmarked.

When you are happy with the plot, use the 'clone current setting' button (upper right button in the plot tab). One of the unique features of this app is that the settings can be stored, enabling reproducible plots. Background on several normalization methods and their application can be found in this blog. Normalization is usually performed to facilitate comparison of different measurements. The web tool offers several options for the normalization of data. For more information on the conversion of spreadsheet data to tidy data see this blog. Direct import of tidy data is also supported.

The data is converted into tidy format to enable plotting with ggplot2. Multiple file upload is supported and can be used to upload data from different conditions that can be individually colored or displayed. The data can be supplied in spreadsheet/long format (e.g. Some background on these dataviz styles can be found in this blog from measurements on multiple objects) the user can plot the data as 'small multiples' or in a heatmap style. To facilitate the display of heterogeneous responses (e.g.

More details about the PlotTwist app can be found in our preprint. The user has full control over the visibility of the raw data and statistics by adjustment of the transparency (alpha). Summary statistics (mean) and inferential statistics (confidence intervals) can be displayed as well.

Several different options for plotting are available lineplot, small multiples and heatmap. The PlotTwist Shiny app plots the data and statistics of continuous data, with a focus on data from timelapse experiments.
